When the cell located strong electric field, transmembrane potential is getting increase by short pulse(1§Á square pulse) and makes whole or destroy(die).

 

Figure 1. Electroporation of cell membrane due to compression when exposed to high intensity electric fields.

(A) in the absence of electric field E = 0
(B) in the presence of electric field E < Ec
(C) in the presence of electric field E > Ec
(D) in the presence of electric field E >> Ec


Figure 2. Square wave pulse generating circuit

 


2. High voltage pulse sterilization system

In order to develope high voltage pulse sterilization technology, we made bench scale high voltage pulse generator to use exponential pulse wave.

 


 

Small scale pilot system

Load voltage

50 kV

Pulse width

1¡­5 §Á

Peak load currents

2000 A

Pulse repetitive rate

3 ¡­ 5000 Hz

Average power in Load

40 kV

 


Fig. 3. Small scale pilot system